Custom Protein Design
Experience
Orla has extensive experience in providing bespoke contract protein design, expression and manufacture, especially for surface applications such as biosensors, biochips, medical devices, biomaterials and diagnostics.
If you are looking to develop new products with immobilised proteins, to improve existing products or implement or improve processes our dedicated team would be happy to discuss your requirements. OrlaSURF is a patented technology platform that is rapidly transferrable and enables simple, efficient, economic and scalable manufacturing. We are open to opportunities for technology out-licensing, collaboration or full-service customisation projects for the use of OrlaSURF technology.
Our experienced team will work with you to understand your protein and surface needs to design, manufacture and deliver the best solution for you. Please send us an enquiry to arrange a meeting or for more information.
I collaborated with Orla on peptide coated surfaces for stem cell growth. Orla are a fantastic company to work with, they showed continued enthusiasm for the project and always contributed intellectually to the project design. They respond rapidly to requests and are honest and straightforward to deal with.
OrlaSURF
Protein Surface Technology (OrlaSURF)
If your product or process requires immobilisation of a protein on a surface with maximum retention of protein function, then OrlaSURF technology is the right solution for you. OrlaSURF has proven advantages for the immobilisation of proteins for a range of applications. It can be used to display small motifs and larger proteins (e.g. binding domains, growth factors, enzymes, antigens). OrlaSURF technology can improve the functionality of immobilised protein compared to direct adsorption or chemical coupling – in former cases the function frequently improved by several orders of magnitude.
We offer a comprehensive service to fuse your protein of interest to the most appropriate partner from the OrlaSURF immobilisation toolkit. This is dependent upon the end use of the protein. In all cases we have a simple, modular workflow with validation and in-process quality checks.
Workflow
Protein design and construction of expression vector
Protein purification and refolding
Quality assurance testing
Evaluation of functions
Our team is on hand to talk to you. Please send us an enquiry to arrange a meeting or for more information.
Protein Engineering
Advanced Protein Engineering
In addition to our specific expertise in the engineering of proteins for surface immobilisation we have many years of experience in the design and production of proteins for other applications. We have created many ‘bespoke’ proteins for clients in a wide variety of application areas.
We can design and purify proteins for many applications. A few examples:
- Antigen presentation
- Mutants/variants e.g. point mutants, deletions, insertions
- Isolation of specific domains
- Fusions of multiple domains
- Single chain variants of multimeric proteins (e.g. scFv)
- Membrane protein fusions
- Fusions with interaction modules e.g. biotinylation sites, leucine zippers etc.
Our team is on hand to talk to you. Please send us an enquiry to arrange a meeting or for more information.
E. coli
E. coli Expression
We have many years of experience in the production of proteins in E. coli; whether as soluble proteins or refolding from inclusion bodies. Our service for production of his-tagged proteins in E. coli is highly competitive and cost effective. We have particular expertise in the production of membrane proteins as inclusion bodies and their subsequent refolding.
We also offer our proprietary TolA-tag expression system, with proven utility in the expression of proteins in the soluble fraction. The TolA-tag has been proved to be particularly useful in the expression of ‘problematic’ proteins that could not be expressed by other methods owing to toxicity to the host cell or other unknown factor.
A simple, modular workflow with validation at every stage ensures confidence in each step of the process. Our workflow is highly flexible and can be easily adapted to your requirements and needs. Although the cost is project-specific and dependent on complexity, we offer a highly competitive pricing structure.
We offer SDS PAGE, Western Blotting and Circular Dichroism analyses as standard. We can carry out many other types of project-specific analyses e.g. Biacore, ELISA, Mass Spec, activity assays etc.
Workflow
Construction of expression clone
Protein expression and purification
Functional / structural analysis
Our team is on hand to talk to you. Please send us an enquiry to arrange a meeting or for more information.
