Cell Culture

In vivo the behaviour of cells is influenced by the surfaces they attach to. Growing cells on flat 2 dimensional plastic surfaces does not replicate the in vivo conditions cells require for optimum results. We offer an ever increasing range of tools that allow cell biologists to mimic in vivo conditions.

Biomimetic Orla Protein coated surfaces

Orla protein coated cell culture surfaces mimic the effects of cell-cell and cell-extracellular matrix interactions.

Features

  • Single use cell culture inserts
  • Coverslip format compatible with immunofluorescene and microscopy studies
  • Free from animal products
  • Maintain cells for prolonged periods of time
  • Tested with a range of cell types
  • Promote cell attachment, growth and differentiation

cell culture

We have a library of proteins that can be used individually or mixed together to provide a huge range of possible surfaces.

Protein

Motifs displayed

Motif source

Orla 1

RGD

Fibronectin

Orla 34

PHSRN

Fibronectin

Orla 62

FHRRIKA

Fibronectin

Orla 35

RGD & PHSRN

Fibronectin

Orla 11

IKVAV

Laminin

Orla 36

YIGSR

Laminin

Orla 37

IKVAV & YIGSR

Laminin

Orla 31

GTPGPQGIAGQRVV

Collagen

Orla 32

MNYYSNS

Collagen

Orla 30

VFDNFVKL

Tenascin C

Orla 51

Nogo 66

Nogo

Orla 18

IgG binding domains

Protein A

Orla 79

IPKASSVPTELSAISTLY & VFDNVKL

BMP-2 & Tenascin C

Orla 80

IPKASSVPTELSAISTLY

BMP-2

OrlaMatrix synthetic 3D scaffold

OrlaMatrix is an easy to handle 3D cell culture system. The 3D micro fibre structure provides a great increase in surface area over 2D cell culture allowing you to culture more cells in an environment that better mimics in vivo conditions. To enhance cell attachment OrlaMatrix is coated with hydroxyapatite.

 Magnified view of OrlaMatrix fibres    OrlaMatrix in 12 well plate    

Advantages

  • Free of animal products
  • 3D structure provides greater surface area
  • 3D structure better mimics in vivo conditions
  • Easy to handle
  • Stable at room temperature
  • available in a range of sizes

More information about Orla cell culture surfaces view our cell culture application note or visit the frequently asked questions section.

For more technical information about our cell culture surfaces or to place an order contact us