Assembling functional proteins on gold-glass surfaces
Orla Protein Technologies’ Industrial CASE student Tim Robson has presented his latest results on the oriented assembly of engineered outer membrane proteins on the surface of immobilised gold nanoparticles. A poster describing this work was received with great interest at last month’s Biophysical Society Annual Meeting in New Orleans.
Orla Protein Technologies have developed a method to generate ordered protein arrays on gold surfaces. This is made possible by combining gold-thiol chemistry with the inherent self-assembling nature of bacterial outer membrane proteins (OMPs). These highly robust protein scaffolds can then be engineered with a multitude of functional domains or motifs which are displayed in an ordered fashion on the monolayer surface. This technology has broad application in analytical devices, diagnostics, cell culture and bioprocessing.
Tim recently developed a simple method for depositing high densities of gold nanoparticles (AuNP) on to borosilicate glass surfaces. He has gone on to demonstrate the highly oriented assembly of engineered outer membrane proteins on the surface of such immobilised gold nanoparticles.
About the Biophysical Society Annual Meeting
Each year, the Biophysical Society Annual Meeting brings together more than 6,000 researchers working in the multidisciplinary fields representing biophysics. With more than 3,600 poster presentations, over 200 exhibits, and more than 20 symposia, the BPS Annual Meeting is the largest meeting of biophysicists in the world. Despite its size, the meeting retains its small-meeting flavor through its subgroup symposia, platform sessions, social activities and committee programs. The 61st Annual Meeting was held at Ernest N. Morial Convention Center in New Orleans, Louisiana.